Two types of microscopes that are frequently employed in biology and medicine are fluorescence microscopes and inverted microscopes. The inverted microscope and fluorescence microscope will be contrasted and compared in this article. It will go over each type of microscope’s optical designs, applications, advantages, and disadvantages.
What Are the Different Optical Designs of Florescence Microscope Or Inverted Microscope?
Different optical designs differentiate fluorescence microscopes from inverted microscopes.
Optical Design of Fluorescence Microscopes
Fluorescence microscopes use a light source to excite fluorescent molecules in the specimen. The longer wavelength light that the stimulated molecules release is subsequently captured by the objective lens and transmitted through a dichroic mirror. The excitation light is reflected and the emission light is transmitted by the dichroic mirror. The remaining excitation light gets blocked after the emission light has gone through an emission filter. The eyepiece or camera is then used to view the filtered emission light. The optical components of a fluorescence microscope are arranged as follows:
- Light source: The light source is commonly an LED lamp or high-intensity arc lamp.
- Eyepiece or camera: Viewing the image of the specimen is done through an eyepiece or camera.
- Condenser: The light from the light source is focused on the specimen by the condenser.
- Dichroic mirror: The dichroic mirror transmits the emission light while reflecting the excitation light.
- Excitation filter: Only the light wavelengths that will excite the fluorescent molecules in the material can pass through the excitation filter.
- Emission filter: Any additional excitation light is blocked by the emission filter.
- Objective lens: The objective lens gathers and magnifies the emission light from the specimen.
Optical Design of Inverted Microscopes
Specimens that are put upside down on the stage are seen using inverted microscopes. This is useful for seeing specimens that are attached to a culture dish’s or petri dish’s bottom. The optical components of an inverted microscope are arranged as follows:
- Light source: The condenser sits above the light source.
- Eyepiece or camera: Above the objective lens is where the eyepiece or camera is placed.
- Objective lens: The objective lens is pointed upward and is situated below the stage.
- Condenser: Above the stage, the condenser is situated and it points downwards.
- Stage: The specimen is set up on the stage.
Comparison of Optical Design of Fluorescence Microscopes and Inverted Microscopes
The position of the light source and objective lens is the main difference between fluorescence and inverted microscopes. The light source and objective lens in a fluorescence microscope are located above and below the stage. The light source and objective lens in an inverted microscope are both above the stage.
The type of light that is used by fluorescence and inverted microscopes is another distinction between the two. Excitation light is used in fluorescence microscopes to light up fluorescent molecules in the specimen, and the specimen is illuminated by transmitted or reflected light in inverted microscopes.
What Are the Different Applications of Florescence Microscope Or Inverted Microscope?
A fluorescence microscope is often used to study living cells. This is because a fluorescence microscope allows for the visualization of specific molecules within cells. For example, a fluorescence microscope can be used to visualize the nucleus, mitochondria, and other cellular organelles. An inverted microscope is often used to study tissue cultures. Tissue cultures are thin layers of cells that are grown in laboratory dishes. The inverted microscope allows for the visualization of tissue cultures without disturbing them.
Applications of Fluorescence microscope
Some specific applications of fluorescence microscope include:
- Studying the structure and function of cells: Fluorescence microscope can be used to visualize specific molecules within cells, such as the nucleus, mitochondria, and other cellular organelles. This can help researchers to understand how cells work and how they respond to different stimuli.
- Studying the development of embryos and organisms: Fluorescence microscope can be used to study the development of embryos and organisms. This can help researchers to understand how organisms develop and how they are affected by different environmental factors.
- Detecting and identifying pathogens: A fluorescence microscope can be used to detect and identify pathogens, such as bacteria and viruses. This is useful for diagnosing diseases and tracking the spread of infections.
Applications of Inverted microscope
Some specific applications of the inverted microscope include:
- Studying cell-cell interactions: An inverted microscope can be used to study cell-cell interactions. This is useful for understanding how cells communicate with each other and how they form tissues and organs.
- Performing micromanipulation: Micromanipulation is the use of specialized tools to manipulate microscopic objects. An inverted microscope is often used to visualize specimens during micromanipulation experiments.
- Studying tissue cultures: Tissue cultures are thin layers of cells that are grown in laboratory dishes. The inverted microscope allows for the visualization of tissue cultures without disturbing them.
What Are the Different Advantages and Disadvantages of Florescence Microscope Or Inverted Microscope?
Compared to an inverted microscope, a fluorescence microscope has several advantages. Higher-resolution images are produced by a fluorescence microscope, which also makes it possible to see certain chemicals inside cells. An inverted microscope is better than a fluorescence microscope in several ways. Compared to a fluorescence microscope, the inverted microscope is less expensive and simpler to use. The inverted microscope cannot see specific molecules within cells and does not produce images with the same level of detail as a fluorescence microscope.
Advantages of Fluorescence microscope
- Specificity: It is possible to use a fluorescence microscope to accurately see particular chemicals within cells.
- Sensitivity: Even tiny amounts of fluorescent molecules can be found using a fluorescence microscope, which has a very high sensitivity.
- High resolution: A fluorescence microscope can produce images of things with incredible resolution.
Disadvantages of Fluorescence microscope
- Requires special fluorescent labels
- Can be phototoxic to cells
Advantages of Inverted microscope
- Versatility: A variety of items, including living cells, tissue cultures, and eggs, can be viewed under inverted microscopes.
- Ease of use: Using an inverted microscope tends to be simple.
- Accessibility: Inverted microscopes are widely used and reasonably priced.
Disadvantages of Inverted microscope
- Lower resolution than fluorescence microscope
- Cannot be used to visualize specific molecules within cells
Inverted microscopes and fluorescence microscopes are two separate types of microscopes with various optical configurations, uses, advantages as well and disadvantages. Inverted microscopes are used to see specimens that are placed inverted on the stage, whereas fluorescence microscopes are used to see fluorescent molecules in specimens. Depending on the individual requirements of the researcher, the ideal kind of microscope for use will vary.